Fertility Laboratories of Colorado
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PUBLICATIONS

Books
   
1. Handbook of In Vitro Fertilization (Second Edition)
Eds. AO Trounson and DK Gardner. CRC Press, Boca Raton. 1999.
   
2.

Seminars in Reproductive Medicine:

Novel Approaches to Assisted Reproduction: In Vitro Maturation of Gametes and Embryos.
Eds. DK Gardner and Z Rosenwaks. Thieme, New York. 2000.

   
3. Textbook of Assisted Reproductive Techniques: Laboratory and Clinical Perspectives
Eds. DK Gardner, A Weissman, C Holwes and Z Shoham. Martin Dunitz Press, London. 2001.
   
4. ART and the Human Blastocyst
Eds. DK Gardner and M Lane. Springer-Verlag, New York. 2001.
   
5. A Laboratory Guide to the Mammalian Embryo.
Eds. DK Gardner, M Lane and AJ Watson. Oxford University Press. 2003.
   
6. Textbook of Assisted Reproductive Techniques:  Laboratory and Clinical Perspectives (Second Edition)
Eds. DK Gardner, A Weissman, C Howles and Z Shoham.  Taylor and Francis Press, London. 2004.
   
7. In Vitro Fertilization:  A Practical Approach
Eds. DK Gardner, Informa Healthcare, New York. 2006.
   
Chapters in Books
   
1. Gardner DK, Lane M, Kouridakis K, and Schoolcraft WB (1997) Complex physiologically based serum-free culture media increase mammalian embryo development. In In Vitro Fertilization and Assisted Reproduction. Eds. V Gomel and Leung PCK. Monduzzi Editoire, Bologna, pp 187-191.
   
2. Gardner DK and Schoolcraft WB (1998) Elimination of high order multiple gestations by blastocyst culture and transfer. in Female Infertility Therapy: Current Practice. Eds. Z. Shoham, C Howles and H Jacobs. Martin Dunitz, London, pp. 267-74.
   
3. Gardner DK (1998) Improving embryo culture and enhancing pregnancy rate. in Female Infertility Therapy: Current Practice. Eds. Z. Shoham, C Howles and H Jacobs. Martin Dunitz, London, pp. 283-99.
 
   
4. Bongso A and Gardner DK (1999) Embryo development. in Handbook of In Vitro Fertilization, Second Edition. eds. A Trounson and DK Gardner. CRC Press, Boca Raton, pp 167-180.
   
5. Bongso A, Sakkas D and Gardner DK (1999) Co-culture of embryos with somatic helper cells. in Handbook of In Vitro Fertilization, Second Edition. eds. A Trounson and DK Gardner. CRC Press, Boca Raton, pp 181-204.
   
6. Gardner DK and Lane M (1999) Embryo culture systems. in Handbook of In Vitro Fertilization, Second Edition. eds. A Trounson and DK Gardner. CRC Press, Boca Raton, pp 205-264.
   
7. Gardner DK and Leese HJ (1999) Assessment of embryo metabolism and viability. in Handbook of In Vitro Fertilization, Second Edition. eds. A Trounson and DK Gardner. CRC Press, Boca Raton, pp 347-372.
   
8. Gardner DK and Schoolcraft WB (1999) In-vitro culture of human blastocysts. in Towards Reproductive Certainty: Fertility and Genetics Beyond 1999. eds. Jansen, R. and Mortimer, D. Parthenon Press, Carnforth, pp 378-388.
   
9. Gardner DK and Lane M (2001) Embryo culture. in Textbook of Assisted Reproductive Technology: Laboratory and Clinical Perspectives. eds. DK Gardner, A Weissman, C Holwes and Z Shoham. Martin Dunitz Press, London. pp 203-222.
   
11. Lane M and Gardner DK (2001) Embryo homeostasis. in ART and the Human Blastocyst. eds. DK Gardner and M Lane. Springer-Verlag, New York. pp. 69-90.
   
12. Gardner DK and Lane M (2001) Culture systems and blastocyst development. in ART and the Human Blastocyst. eds. DK Gardner and M Lane. Springer-Verlag, New York. pp. 118-143.
   
13. Gardner DK and Lane M (2002) Development of viable mammalian embryos in vitro: Evolution of sequential media. in Principles of Cloning. eds. J Cibelli, R Lanza, K Campbell, and MD West. Academic Press San Diego. pp. 187-213.
   
14. Gardner DK (2002) Embryo metabolism. in Reproduccion Humana. eds. J Remohi, A Pellicer, C Simon and J Navarro. pp 413-420.
   
15. Gardner DK and Lane M (2002) Culture media for the human embryo. In Biotechnology of Human Reproduction. Ed. A Revelli, I Tur-Kaspa, JG Holte, M Massobrio. Parthenon Press. pp 181-199.
   
16. Gardner DK and Lane M (2003) Metabolic requirements during preimplantation development and the formulation of culture media. In An Atlas of Human Blastocysts. Eds. LL Veeck and N Zaninovic. Parthenon Publishing. pp 41-60.
   
17. Lane M and Gardner DK (2004) Preparation of gametes, in vitro maturation. In vitro fertilization and embryo recovery. In A Laboratory Guide to the Mammalian Embryo. Eds. DK Gardner, M Lane and AJ Watson. Oxford University Press. pp 24-40.
   
18. Gardner DK and Lane M (2004) Culture of the mammalian preimplantation embryo. In vitro fertilization and embryo recovery. In A Laboratory Guide to the Mammalian Embryo. Eds. DK Gardner, M Lane and AJ Watson. Oxford University Press. pp 41-61.
   
19. Sakkas D, Lane and Gardner DK (2004) Assessment of embryo development and viability. In vitro fertilization and embryo recovery. In A Laboratory Guide to the Mammalian Embryo. Eds. DK Gardner, M Lane and AJ Watson. Oxford University Press. pp 62-75.
   
20. Lane M, Baltz J and Gardner DK (2004) Analysis of intracellular ions: pH and calcium. In vitro fertilization and embryo recovery. In A Laboratory Guide to the Mammalian Embryo. Eds. DK Gardner, M Lane and AJ Watson. Oxford University Press. pp 125-138.
   
21. Gardner DK and Lane M (2004) Nutrient uptake and metabolite production and enzyme activity and regulation. In vitro fertilization and embryo recovery. In A Laboratory Guide to the Mammalian Embryo. Eds. DK Gardner, M Lane and AJ Watson. Oxford University Press. pp 139-153.
   
22. Gardner DK and Lane M (2004) Embryo culture. In Textbook of Assisted Reproductive Technology: Laboratory and Clinical Perspectives, Second Edition. Eds. DK Gardner, A Weissman, C Howles and Z Shoham. Martin Dunitz Press, London. pp 211-234.
   
23. Sakkas D and Gardner DK (2004) Assessment of embryo viability. In Textbook of Assisted Reproductive Technology: Laboratory and Clinical Perspectives, Second Edition. Eds. DK Gardner, A Weissman, C Holwes and Z Shoham. Martin Dunitz Press, London. pp 235-245.
   
24. Gardner DK and Lane M (2006) Embryo culture systems. In IVF a Practical Approach. Ed. DK Gardner. Infoma Healthcare, New York. pp 221-282.
   
25. Gardner DK (2006) Non-invasive assessment of single cell metabolism. In Single Cell Diagnostics Ed. AR Thornhill, Methods in Molecular Medicine Series. Series Editor: John Walker. Humana Press, London. (in press).
   
26. Gardner DK (2006) Human embryonic development in vitro. In Human IVM. Ed. SL Tan. Taylor & Francis, New York.
   
27. Gardner DK, Stevens J, Sheehan CB and Schoolcraft WB (2006) Morphological assessment of the human blastocyst. In Analysis of the Human Embryo. Eds. K Elder and J Cohen. Taylor & Francis, New York. (in press).
   
Publications in Refereed Journals
   
1. Gardner DK and Lane M (1997) Culture and selection of viable blastocysts: a feasible proposition for human IVF? Hum. Reprod. Update 3: 367-382.
   
2. Gardner DK, Vella P, Lane M, Wagely L, Schlenker T and Schoolcraft WB (1998) Culture and transfer of human blastocysts increases implantation rates and reduces the need for multiple embryo transfers. Fertil. Steril. 69: 84-88.
   
3. Gardner DK (1998) Changes in requirements and utilization of nutrients during mammalian preimplantation embryo development and their significance in embryo culture. Theriogenology 49: 83-102.
   
4. Lane M and Gardner DK (1998) Amino acids and vitamins prevent culture-induced metabolic perturbations and associated loss of viability of mouse blastocysts. Hum. Reprod. 13: 991-997.
   
5. Gardner DK (1998) Development of serum-free media for the culture and transfer of human blastocysts. Hum. Reprod. 13: Supplement 4: 218-225.
   
6. Gardner DK and Schoolcraft WB (1998) Human embryo viability: what determines developmental potential and can it be assessed? J. Assist. Reprod. Genet. 15: 455-458.
   
7. Edwards LJ, Williams DA and Gardner DK (1998) Intracellular pH of the preimplantation mouse embryo: effects of extracellular pH and weak acids. Mol. Reprod. Devel. 50: 434-442.
   
8. Gardner DK, Schoolcraft WB, Wagley L, Schlenker T, Stevens J and Hesla J (1998) A prospective randomized trial of blastocyst culture and transfer in in vitro fertilization. Hum. Reprod.13: 3434-3440.
   
9. Gardner DK and Schoolcraft WB (1998) No longer neglected: the human blastocyst. Hum. Reprod.13: 3289-3292.
   
10. Edwards LJ, Williams DA and Gardner DK (1998) Intracellular pH of the preimplantation mouse embryo: amino acids act as buffers of intracellular pH. Hum. Reprod. 13: 3441-3448.
   
11. Gardner DK and Schoolcraft WB (1999) Culture and transfer of human blastocysts. Curr. Op. Obs. Gyn. 11: 307-311.
   
12. Steeves TE and Gardner DK (1999) Temporal and differential effects of amino acids on bovine embryo development in culture. Biol. Reprod. 61: 731-740
   
13. Steeves TE, Gardner DK, Zuelke KA, Squires TS and Fry RC (1999) In vitro development and nutrient uptake by embryos derived from oocytes of pre-pubertal and adult cows. Mol. Reprod. Devel. 54: 49-56.
   
14. Steeves TE and Gardner DK (1999) Metabolism of glucose, pyruvate and glutamine during the maturation of oocytes derived from pre-pubertal and adult cows. Mol. Reprod. Devel. 54: 92-101.
   
15. Gardner DK, Rodriegez-Martinez H and Lane M (1999) Fetal development after transfer is increased by replacing protein with the glycosaminoglycan hyaluronate for embryo culture and transfer in the mouse. Hum. Reprod. 14: 2575-2580.
   
16. Schoolcraft WB, Gardner DK, Lane M, Schlenker T, Hamilton F and Meldrum DR (1999) Blastocyst culture and transfer: analysis of results and parameters affecting outcome in two in vitro fertilization programs. Fertil. Steril. 72: 604-609.
   
17. Marek D, Langley M, Gardner DK, Confer N, Doody KM and Doody KJ (1999) Introduction of blastocyst culture and transfer for all patients in an in vitro fertilization program. Fertil. Steril. 72: 1035-1040.
   
18. Lane M, Schoolcraft WB and Gardner DK (1999) Vitrification of mouse and human blastocysts using a novel cryoloop container-less technique. Fertil. Steril. 72: 1073-1078.
   
19. Lane M and Gardner DK (2000) Lactate regulates pyruvate uptake and metabolism in the preimplantation mouse embryo. Biol. Reprod. 62: 16-22.
   
20. Gardner DK, Lane M, Stevens J, Schlenker T and Schoolcraft WB (2000) Blastocyst score affects implantation and pregnancy outcome: towards a single blastocyst transfer. Fertil. Steril. 73: 1155-1158.
   
21. Schoolcraft WB and Gardner DK (2000) Blastocyst culture and transfer increases the efficiency of oocyte donation. Fertil. Sertil. 74: 482-486.
   
22. Gardner DK, Lane M and Schoolcraft WB (2000) Culture and transfer of viable blastocysts: A feasible proposition for human IVF. Hum. Reprod. 15: Supplement 6: 9-23.
   
23. Gardner DK (2000) Blastocyst culture: toward single embryo transfers. Hum. Fertil. 3: 229-237.
   
24. Gardner DK, Lane MW and Lane M (2000) EDTA stimulates cleavage stage bovine embryo development in culture but inhibits blastocyst development and differentiation. Mol. Reprod. Devel. 57:256-261.
   
25. Gardner DK, Pool TB and Lane M (2000) Embryo nutrition and energy metabolism and its relationship to embryo growth, differentiation and viability. Semin. Reprod. Med. 18:205-218.
   
26. Lane M and Gardner DK (2000) Regulation of ionic homeostasis by mammalian embryos. Semin. Reprod. Med. 18:195-204.
   
27. Lane M and Gardner DK (2001) Vitrification of mouse oocytes using a nylon loop. Mol. Reprod. Devel. 58: 342-347.
   
28. Langley MT, Marek DM, Gardner DK, Confer N, Doody KM and Doody KJ (2001) Extended embryo culture in human assisted reproduction treatments. Hum. Reprod. 16: 902-908.
   
29. Schoolcraft WB, Surrey ES and Gardner DK (2001) Embryo transfer: techniques and variables affecting success Fertil. Steril. 76: 863-870.
   
30. Lane M and Gardner DK (2001) Inhibiting 3-phosphoglycerate kinase by EDTA stimulates the development of the cleavage stage mouse embryo. Mol. Reprod. Dev. 60: 233-240.
   
31. Lane M, Hooper K and Gardner DK (2001) Effect of essential amino acids on mouse embryo viability and ammonium production. J. Assist. Reprod. Genet. 18: 519-525.
   
32. Schoolcraft WB and Gardner DK (2001) Blastocyst versus day 2 or 3 transfer. Semin. Reprod. Med. 19: 259-268.
   
33. Gardner DK (2001) Improving implantation rates in IVF. Infertility and Reprod. Med. Clinics of North America. 12, 403-246.
   
34. Gardner DK, Lane M, Stevens J and Schoolcraft WB (2001) Non-invasive assessment of human nutrient consumption as a measure of developmental potential. Fertil. Steril. 76:1175-1180.
   
35. Gardner DK, Lane M and Schoolcraft WB (2002) Physiology and culture of the human blastocyst. J. Reprod. Immunol. 55: 85-100.
   
36. Reed ML, Lane M, Gardner DK, Jensen NL and Thompson J (2002) Vitrification of human blastocysts using the cyroloop method: successful clinical application and birth of offspring. J. Assist. Reprod. Genet.19:304-306.
   
37. Lane M, Maybach JM and Gardner DK (2002) Addition of ascorbate during cryopreservation stimulates subsequent embryo development. Hum. Reprod. 17:2686-2693.
   
38. Gardner DK, Lane M, Stevens J and Schoolcraft WB (2003) Changing the start temperature and cooling rate in a slow freezing protocol increases human blastocyst viability. Fertil. Steril. 79: 401-410.
   
39. Bavister BD, Kinsey, DL, Lane M and Gardner DK (2003) Recombinant human albumin supports hamster in vitro fertilization. Hum. Reprod. 18:113-116.
   
40. Lane M, Maybach JM, Hooper K, Hasler JF and Gardner DK (2003) Cryosurvival and development of bovine blastocysts are enhanced by culture in recombinant albumin and hyaluronan. Mol. Reprod. Dev. 64:70-78.
   
41. Lane M, Gardner DK, Hasler MJ and Hasler JF (2003) Use of G1.2/G2.2 media for commercial bovine embryo culture: equivalent development and pregnancy rates compared to co-culture. Theriogenology 60: 407-419.
   
42. Gardner DK, Lane M (2003) Towards a single embryo transfer. Reprod. Biomed. Online 6: 470-481.
   
43. Gardner DK and Lane M (2003) Blastocyst transfer. Clin. Obstet. Gyn. 46: 231-238.
   
44. Gardner DK and Sakkas D (2003) Assessment of embryo viability: the ability to select a single embryo for transfer. Placenta Suppl B: S5-S12.
   
45. Lane and Gardner DK (2003) Ammonium induces aberrant blastocyst differentiation, metabolism, pH regulation, gene expression and subsequently alters fetal development in the mouse. Biol. Reprod. 69:1109-1117.
   
46. Adashi EY, Barri PN, Berkowitz R, Braude P, Bryan E, Carr J, Cohen J, Collins J, Devroey P, Frydman R, Gardner DK, Germond M, Gerris J, Gianaroli L, Hamberger L, Howles C, Jones H Jr, Lunenfeld B, Pope A, Reynolds M, Rosenwaks Z, Shieve LA, Serour GI, Shenfield F, Templeton A, van Steirteghem A, Veeck L, Wennerholm UB (2003) Infertility therapy-associated multiple pregnancies (births): an ongoing epidemic. Reprod Biomed Online 7:515-542.
   
47. Gardner DK, Surrey E, Minjarez D, Leitz A, Stevens J, Schoolcraft WB (2004) Single blastocyst transfer: a prospective randomized trial. Fert. Steril. 81: 551-555.
   
48. Seli E, Gardner DK, Schoolcraft WB, Moffatt O and Sakkas D (2004) Extent of nuclear DNA damage in ejaculated spermatozoa impacts on blastocyst development after in vitro fertilization. Fert. Steril. 82: 378-383.
   
49. Karagenc L, Lane M and Gardner DK (2004) Oestradiol, cyclodextrin-encapsulated 17 beta-oestradiol and the oestradiol solubilizer 2-hydroxypropyl-beta-cyclodextrin all impair preimplantation mouse embryo development. Rep. Biomed. Online. 9: 280-286.
   
50. Gardner DK, Reed L, Linck D, Sheehan C and Lane M (2005) Quality control in human in vitro fertilization. Sem. Reprod. Med. 23, 319-324.
   
51. Gardner DK and Lane M (2005) Ex-vivo early embryo development and effects on gene expression and imprinting. Reprod. Fertil. Dev. 17:361-70.
   
52. Lane M and Gardner DK (2005) Understanding cellular disruptions during early embryo development that perturb viability and fetal development. Reprod. Fertil. Dev. 17:371-8.
   
53. Karagenc L, Lane M and Gardner DK (2005) Granulocyte macrophage colony stimulating factor stimulates mouse blastocyst inner cell mass development only when media lack human serum albumin. Rep. Biomed. Online 10: 511–518
   
54. Lane M and Gardner DK (2005) Mitochondrial malate-aspartate shuttle regulates mouse embryo nutrient consumption. J. Biol. Chem. 280:18361-18367.
   
55. Sakkas D and Gardner DK (2005) Noninvasive methods to assess embryo quality. Curr Opin Obstet Gynecol. 17:283-288.
   
56. Preis KA, Seidel G Jr and Gardner DK (2005) Metabolic markers of developmental competence for in vitro-matured mouse oocytes. Reproduction 130: 475-483.
   
57. Walker DL, Gardner DK, Lane M, Tummon IS, Session DR, Thornhill AR (2005) Cryosystem assessment by glucose uptake of blastocysts. Reprod. Biomed. Online 11: 601-607.
   
58. Katz-Jaffe M, Linck DW, Schoolcraft WB and Gardner DK (2005) A proteomic analysis of mammalian preimplantation embryonic development. Reproduction 130: 899-905.
   
59. Larman MG, Sheehan CB and Gardner DK (2006) Calcium-free vitrification reduces cryoprotectant-induced zona pellucida hardening and increases fertilization rates in mouse oocytes. Reproduction 131: 56-61.
   
60. Katz-Jaffe M, Gardner DK and Schoolcraft WB (2006) Proteomic analysis of individual human embryos to identify novel biomarkers of development and viability. Fertil. Steril. 85: 101-107.
   
61. Larman MG, Sheehan C and Gardner DK (2006) Vitrification of mouse pronuclear oocytes with no direct liquid nitrogen contact. Rep. Biomed. Online 12; 66-69.
   
62. Gardner DK and Balaban B (2006) Choosing between day 3 and day 5 embryo transfers. Clin. Obstet. Gyn. 49: 85-92.
   
63. Gibbons J, Hewitt E and Gardner DK (2006) Effects of oxygen tension on the establishment, and lactate dehydrogenase activity, of murine embryonic stem cells. Cloning Stem Cells 8: 117-122.
   
64. Katz-Jaffe M, Schoolcraft WB and Gardner DK (2006) Analysis of protein expression (secretome) by human and mouse preimplantation embryos. Fertil. Steril. 86: 678-685.
   
65. Sheehan C, Lane M and Gardner DK, (2006) The CryoLoop facilitates re-vitrification of embryos at four successive stages of development without impairing embryo growth. Hum. Reprod. 21: 2978-2984.
   
66. Lane M and Gardner DK (2006) Embryo culture medium – which is best? Clinical Obstet Gyn. (in press).
   
67. Gardner DK, Sheehan C, Rienzi L, Katz-Jaffe M and Larman MG (2007) Analysis of oocyte physiology to improve cryopreservation procedures. Theriogenology 67: 64-72.
   
68. Larman M, Katz-Jaffe MG, Sheehan CB and Gardner DK (2007) 1,2-propanediol and the type of cryopreservation procedure adversely affect mouse oocyte physiology. Hum. Reprod. (in press).
   
69. Preis KA, Seidel G Jr and Gardner DK (2007) Reduced oxygen concentration improves the developmental competence of mouse oocytes following in vitro maturation. Mol. Reprod. Dev. (in press).
   
   
 

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